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To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig

Duration : Treatment can take 1 to 2 hours

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One-carbon (1-C) metabolism requires the proper operation of folate and vitamin B12

HGF can upregulate the transcription factor Ets-1, thus enhancing the expression of vascular endothelial growth factors (VEGFs) (Tomita et al., 2003)